GIN, Genome Inspector, was devised to design primers within microbial species or genus as broad as possible, for PCR gene-screening projects. It uses BLAST to find DNA sequences highly similar to the gene or sequence chosen by the user, in complete sequenced microbial genomes (GenBank format only). When an identical match is found, it draws a circular map showing the position of the highest score match, registers its position on a table and shows the BLAST alignments. The sequences found by BLAST will be used to perform a multiple sequence comparison by log-expectation (MUSCLE). The user will easily evaluate if the conserved found sequences will give, or not, broad range primers depending on the % of genomes in the multiple alignment.
